发明名称 |
AMPLIFYING THE EXPRESSION OF RECOMBINANT DNA PRODUCTS |
摘要 |
Amplified expression of recombinant DNA products is achieved in hosts expressing protease that cleave at multi-basic amino acid residues. To this end, wild-type genes encoding the desired protein products are mutated by substituting codons or eliminating codons encoding multi-basic amino acid residues while maintaining the activity of the expressed protein product. Mutation of the desired gene can be conveniently carried out by site-specific in vitro mutagenisis. |
申请公布号 |
ZA8605651(B) |
申请公布日期 |
1987.04.29 |
申请号 |
ZA19860005651 |
申请日期 |
1986.07.29 |
申请人 |
IMMUNEX CORPORATION |
发明人 |
MICHAEL C. DEELEY;STEVEN D. GIMPEL;VIRGINIA L. PRICE |
分类号 |
C12N15/09;A61K35/74;A61K38/16;C07K14/00;C07K14/52;C07K14/53;C07K14/535;C12N1/16;C12N15/00;C12N15/10;C12N15/27;C12P21/00;C12P21/02;C12R1/865 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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