Method for the detection of a polypeptide subunit in the presence of a quaternary protein containing the subunit.

摘要

A method for the determination of a free protein subunit of a quaternary protein in a sample, which comprises:
<UnorderedLists id="ula01" listStyle="none"><ListItem>(a) contacting a sample with a first immunological binding partner which is or will be bound to a carrier, wherein the first immunological binding partner binds eiptopic determinants bindable only on the free protein subunit;</ListItem><ListItem>(b) incubating the components of step (a) for a period of time and under conditions sufficient to form an immune complex between the free protein subunit, the first immunological binding partner, and the carrier;</ListItem><ListItem>(c) separating the carrier of step (b) from the sample;</ListItem><ListItem>(d) adding to the carrier of step (c), a detectably-labeled second immunological binding partner, wherein the second immunological binding partner binds epitopic determinants bindable on both the free protein subunit and the quaternary protein; and</ListItem><ListItem>(e) determining the detectably-labeled second immunological binding partner in the carrier or in liquid phase.</ListItem></UnorderedLists>