| 摘要 |
PURPOSE:To obtain a dermal medical agent effective against liver-spot skin roughening, dermatitis, etc., by cultivating novel Trichosporon kashiwayama strain (FERM-P No. 4821) under specific conditions and collecting the germ-free supernatant liquid of the above-mentioned culture fluid. CONSTITUTION:Trichosporon kashiwayama strain (hereinafter abbreviated to kashiwayama strain) is preferably cultivated in a yeast culture medium at 4.0-6.0pH consisting of 0.3 wt./vol.% glucose, 0.5 wt./vol.% skimmilk and 0.05 wt./vol.% yeast extract at 20-30 deg.C for 3-7 days. The resultant culture fluid is aseptically filtered through a membrane filter, etc., and the germ-free supernatant liquid is concentrated and dried at any time to give the aimed dermal medical agent. The fermentation product of the kashiwayama strain is found to have remarkable medical effect on the skin and the effective against liver-spot, skin roughening, fine wrinkles, contact-type dermatitis, burn, etc. The agent has no irritancy and contains no antibiotic substance. |
