Pure alkaline phosphatase, its preparation and use

摘要

The enzyme alkaline phosphatase having the following properties: (i) molecular weight: approximately 80 000 with 2 subunits of 40 000 (iii) activator: Mg++ inhibitor: EDTA (iii) thermal stability: 45 DEG C. or below (iv) optimum temperature: 35 DEG -45 DEG C. (v) pH stability: 6-11 (vi) pH optimum: 10 (vii) specific activity: 3000-5000 units/mg (viii) isoelectric range: between pH 5.0 and 6.0. An antibody-enzyme conjugate process for purifying alkaline phosphatase and a reagent test kit using the purified alkaline phosphatase are also disclosed.