发明名称 |
Purification and activation of proteins from insoluble inclusion bodies. |
摘要 |
<p>Purification and activation of proteins produced in transformant microorganisms as insoluble, biologically inactive inclusion bodies can be effected by solubilizing the inclusion bodies in SDS; dialyzing or refrigerating the protein solution to remove a portion of the SDS; chromatographing the protein solution on an ion-retardation resin to remove the remaining SDS from the protein; and chromatographing the protein solution thus obtained on an anion-exchange resin. </p> |
申请公布号 |
EP0218374(A1) |
申请公布日期 |
1987.04.15 |
申请号 |
EP19860306871 |
申请日期 |
1986.09.05 |
申请人 |
INTERNATIONAL MINERALS AND CHEMICAL CORPORATION |
发明人 |
RAUSCH, STEVEN K.;MENG, HSI |
分类号 |
C12P21/00;C07K1/18;C07K1/36;C07K7/22;C07K14/575;C07K14/61;C12N15/09;C12P21/02;(IPC1-7):C12N15/00;C07K3/12;C07K3/28 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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