| 摘要 |
<p>One amino acid residue in a protein chain is replaced so as to produce a modified protein. Initially, an mRNA molecule capable of producing an unmodified protein of the type it is desired to modify is provided. Next the codon along the mRNA chain which ordinarily accepts the anti-codon corresponding to a first amino acid which is to be replaced with a selected second amino acid is determined. The tRNA which ordinarily brings the first amino acid to that site is modified to carry the second amino acid instead. The modified protein is then formed by translation techniques in the presence of the modified tRNA, whereby the second amino acid is incorporated in the protein chain in place of the first amino acid.</p> |
