摘要 |
<p>PURPOSE:To multiply an animal cell at a high density, by the presence of a polyglycol in a culture fluid for cultivating the animal cell in a cultivation tank in a suspension state in a cultivation tank. CONSTITUTION:A polyglycol is added to a culture fluid for cultivating an animal cell in a suspended state in a culture fluid. A water-soluble homopolymer or copolymer having 200-20,000, preferably 1,000-14,000 is used as the polyglycol and added to the suspension to give 0.001-5g/l, preferably 0.01-0.1g/l concentration. Thereby, the animal cell can be cultivated at >=10<6> cells/ml density.</p> |