| 摘要 |
PURPOSE:To efficiently collect L-isoleucine produce and accumulate in a culture, by cultivating a microorganism, obtained by protoplastic fusion, having ability to produce L-isoleucine and belonging to the genus Corynebacterium in a culture medium. CONSTITUTION:A protoplastic strain, e.g. Corynebacterium glutamicum H-3859 (FERM-P No.849) or Corynebacterium glutamicum H-3860 (FERM-P No.850), obtained by protoplastic fusion between a microorganism having the ability to produce L-isoleucine and a microorganism having the ability to produce an amino acid which is a principal fermentation product other than the L- isoleucine is cultivated in a culture medium and the aimed L-isoleucine is collected from the resultant culture. |
