Yeast promoter and method of producing heterologous proteins.

摘要

A DNA sequence comprising the region of the yeast repressible acid phosphatase (PHO5) promoter which covers the 173 base pairs directly upstream from the initiation codon for the PHO5 protein and a method of producing heterologous proteins using the above DNA sequence as the promoter are provided. The above miniaturized modified yeast promoter enables efficient expression of physiologically active substances in yeasts, providing a miniaturized modified PHO5 prmoter so far never taught, and facilitates recombinant DNA procedures. The above promoter also enables use of the PHO 5 promoter in phosphate-supplemented media and production of physiologically active substances in more convenient medium systems.