| 摘要 |
A process for producing a desired alpha-amino acid, AAd, or a derivative thereof. The process comprises: (a) reacting a first alpha-amino acid, AANH with a first alpha-keto acid, KAt, and a second alpha-keto acid, KApre, in the presence of a first transaminase enzyme and a second transaminase enzyme to produce (i) the desired alpha-amino acid, AAd and (ii) a third alpha-keto acid, KAprod: and (b) removing KAprod from the other keto acids, amino acids and enzymes wherein AAd and KApre, AAt and KAt, and AANH and KAprod are interconvertible, respectively, by amino group transfer. The first transaminase efficiently catalyzes reaction (i), but not reaction (ii) and the second transaminase efficiently catalyzes reaction (ii) but not reaction (i): In one embodiment KAprod is continuously removed for the reaction mixture thus driving the overall reaction to completion. |
