A method of extracting nucleic acids from cells.

摘要

A method of extracting nucleic acids from cells without the use of centrifugation in which cells are treated with proteinase K in the presence of a lysis buffer having a high concentration of salt. The lysate is mixed with a phenol-based solvent system, thereby creating an emulsion which is heated to a temperature of at least 35 DEG C to promote phase separation. The rate of phase separation is also enhanced by increasing the surface area of the emulsion. Once the phase separation is complete, the lower organic phase is removed and the upper aqueous phase is repeatedly extracted with the phenol-based solvent a preselected number of times, and is finally extracted using chloroform. The remaining aqueous phase is then dialyzed to further purify the remaining nucleic acid solution.