CONTINUOUS MASS CULTURE OF ANCHORAGE DEPENDENT CELL

摘要

PURPOSE:To enable the continuation of culture under a definite culture condition for a long period and to obtain the product in high yield, by packing a column with a fibrous carrier, attaching and immobilizing anchorage dependent cells to the carrier and circulating the medium through the column from the bottom upward. CONSTITUTION:A fibrous carrier 2 is packed in the column 1 and anchorage dependent cells are attached and immobilized to the carrier. A medium is circulated through the column 1 from the bottom upward. The cultivation carrier is preferably a fibrous carrier having a length of >=10mm. The anchorage dependent cell is preferably fibroblast.