发明名称 |
Methods for the in vitro detection and identification of unknown pathogens or genetic entities |
摘要 |
Methods for the detection and identification of unknown pathogens or genetic entities from samples suspected of containing the same involve the use of novel wicking techniques whereby sample DNA/RNA becomes readily available on a porous, inert, positively charged support in single stranded form for hybridization with a hybridization probe having a nucleotide sequence substantially complementary to a nucleotide sequence of the sample DNA/RNA. Such methods permit the detection and identification of unknown pathogens and genetic entities in a shorter period of time than that required by conventional methods. Lysis solutions for rapidly lysing bacterial cells without the aid of enzymes are also disclosed.
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申请公布号 |
US4652517(A) |
申请公布日期 |
1987.03.24 |
申请号 |
US19840619286 |
申请日期 |
1984.06.11 |
申请人 |
DIAGNOSTIC RESEARCH LIMITED PARTNERSHIP |
发明人 |
SCHOLL, DAVID R.;JOLLICK, JOSEPH D. |
分类号 |
G01N33/50;C12N1/06;C12N1/20;C12N15/00;C12N15/09;C12N15/10;C12Q1/04;C12Q1/68;G01N33/58;(IPC1-7):C12Q1/70 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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