| 摘要 |
A method and device for performing comparative assay of binding and bindable substances such as antibodies and antigens. The device includes a circulation conduit for continuously conveying a carrier fluid between a support zone therein and a detection zone therein. A competitive binding substance is fixed in the support zone and is capable of competitively binding a test substance and an analytical detection substance such that changes in concentration of the test substance in the carrier fluid responsively cause changes of concentration of analytical detection substance in the carrier fluid. Each of the analytical detection substance (antigen-fluorochrome, for example) and the test substance (antigen, for example) are bound to the binding substance (monoclonal antibody, for example) in proportion to the concentration of the test substance in the carrier fluid. A detection means is provided for detecting changes in the concentration of the analytical detection substance as the carrier fluid is conveyed through the detection zone. Preferably, the detection means comprises a fluorescent detector detecting fluorescent activity produced by the fluorochrome detection substance. The fluorescent detector includes a comparator which compares the degree of fluorescent activity in a lo-flow tube compared to a hi-flow tube. Changes in concentration of the test substance produce a variation in the fluorescent activity in the hi-flow tube compared to the lo-flow tube. The method includes introducing a test substance into the carrier fluid of a device of the type described above. A comparative assay is performed by detecting changes in the concentration of the analytical detection substance produced by the introduction of the test substance.
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