| 摘要 |
Food was pasteurized by using fatty acid having no biohazards against humans and also inhibiting the growth of microbes. Thus, 0.1ml of the 2nd stage broth of Bacillus subtilis var. niger 4830 grown on the medium contg. (NH4)2SO4 2, KH2PO4 4, K2HPO4 4, Na-citrate 1, MSG 3, glucose 5, and MgSO4 0.2% was inoculated into 8.9ml of the above medium and cultivated on the Bioscanner OT-BS12 at 610nm until exponential phase. 1ml of each 0.3mM lauric acid and 0.05mM L-Cys was added into the broth. 0.2ml of broth was sampled from cell aseptically at time intervals and the cell No. was tested by using the plate dilution tech. The cell no. (3.0 x 10/ml) was much smaller than that(4.8 x 1011/ml) of the control after 2hr of addn.
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