| 摘要 |
PURPOSE:To continuously separate electric charge materials having specific isoelectric points and mol.wt. by disposing fractionation membranes having different pore sizes to the inside of a migration chamber in the direction where a DC voltage is impressed and providing outlet nozzles to chamber units at the bottom end of the liquid flow of the migration chamber. CONSTITUTION:The migration chamber 2 of a flow device body 1 is segmented to three chambers 2a, 2b, 2c by the fractionation membranes 5a, 5b which make fractionation according to the mol.wt. Electrodes 6a, 6b are respectively installed in electrode chambers 3a, 3b partitioned by a semipermeable membrane 4. The inlet and outlet nozzles 7a, 7b, 7c, 8a, 8b, 8c are respectively provided to the top and bottom ends of the migration chambers 2a, 2b, 2c. The mol.wt. to be held by the respective fractionation membranes is set at a prescribed value and the pH of a buffer soln. is adjusted to a prescribed value in the case of separating the specific protein from a mixture composed of proteins having respectively specific isoelectric points and mol.wt., then the specific protein moves to the specific electrode side with the relation between the isoelectric points and pH and is taken out of any of the nozzles 8a-8c. |
