Cloning systems for corynebacterium

摘要

The invention is a method for obtaining a chimeric plasmid which can serve as a vector for inserting one or more foreign or native genes into Corynebacterium and a second species of bacteria. The plasmid is the ligation product of plasmid DNA from Corynebacterium as well as plasmid DNA from a non-Corynebacterium species of bacteria. Also disclosed is a method for forming protoplasts from Corynebacterium organisms and transforming the protoplasts with vectors, e.g., the chimeric plasmids of the invention.