| 摘要 |
<p>There is disclosed a perfusable cell culture device capable of expansion and maintenance of biological cells, including mammalian, microbial, plant and insect cells in culture. The device comprises an upper and lower polymeric film layer (8 and 3) and a gas and liquid permeable flow divider membrane (1). The flow divider membrane (1) is affixed between the upper and lower polymeric layers (8 and 3) and to the lower polymeric layer (3) in a manner providing channels (6) for fluid distribution of culture medium (11) which is perfused at a controlled rate, uniformly across the flow divider membranes (1) and to a bed of cells (10) dispersed on the flow divider membrane (1).</p> |
