| 摘要 |
PURPOSE:To produce expensive vitamin B1 easily and efficiently by the use of inexpensive methanol as a carbon source, by subjecting a bacterium belonging to the genus Eu bacterium, etc., capable of assimilating methanol as a carbon source and producing vitamin B12 and a denitrifying bacteria capable of decomposing an organic acid to mixed culture. CONSTITUTION:A bacterium belonging to the genus Eu bacterium or Butyribacterium, capable of producing vitamin B12 and a denitrifying bacterium capable of decomposing an organic acid such as acetic acid and/or butyric acid is subjected to mixed culture in the presence of methanol. Consequently, the organic acid formed and accumulated in the culture solution once is decomposed with the denitrifying bacterium so the concentration of the organic acid in the culture solution is reduced to < concentration to inhibit growth of the bacterium capable of producing vitamin B12. Therefore, the mold concentration is further improved while keeping the high content of vitamin B12 of the bacterium capable of producing vitamin B12 so productivity of vitamin B12 can be improved. |
