发明名称 |
Specific binding assay technique |
摘要 |
The present invention relates to a simple and accurate method to be applied in immunoassay technique for separating the free ligand from the antibody-bound ligand. The method involves the use of a solvent extraction operation which at equilibrium provides the formation of two distinct phases the free- and bound-fraction. The solvent to be utilized should be slightly water miscible or completely water immiscible, having the property of marked selective extraction power toward the gamma-labelled constituent to be determined. The method is applicable in a liquid-liquid system, a solid-liquid system and solid-solid system. The method is useful for radioimmunoassay, free radical assay or metallo-immunoassay, being most versatile having also the advantage that as a result of the free ligand being extracted into the solvent, the determination of the quantity of gamma-labelled substance in the free and/or bound fraction compares very favorably with the known prior art methods in immunoassays.
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申请公布号 |
US4645747(A) |
申请公布日期 |
1987.02.24 |
申请号 |
US19830470334 |
申请日期 |
1983.02.28 |
申请人 |
TECHNION RESEARCH & DEVELOPMENT FOUNDATION, LTD. |
发明人 |
CAIS, MICHAEL;SHIMONI, MOSHE;BANIEL, ABRAHAM |
分类号 |
G01N33/537;(IPC1-7):G01N33/537;G01N33/538 |
主分类号 |
G01N33/537 |
代理机构 |
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主权项 |
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地址 |
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