METHOD FOR MEASURING RETICULOCYTE BY FLOW SIGHT METER

摘要

PURPOSE:To attain to enhance the measuring accuracy of a reticulocyte, by making the amount to be used of a reagent containing auramine O more excessive than the amount precipitating RNA in a blood specimen. CONSTITUTION:When a corpuscle measuring reagent containing auramine O is mixed with an anticoagulant whole blood specimen, the auramine dye vividly stains a corpuscle containing a reticulocyte and is bonded to RNA in a cell to precipitate RNA subjected to fluorescent staining. The specimen stained with the corpuscle measuring specimen used in an amount made more excessive than the amount precipitating RNA in the blood specimen is irradiated with light of a purple - blue wavelength region to measure fluorescence from the specimen. By this method, measuring accuracy can be enhanced even with respect to a corpuscle specimen low in the concn. of a corpuscle containing a reticulocyte or having a low reticulocyte ratio.