发明名称 |
Methods for calibrating chemical assays |
摘要 |
Methods are disclosed for both internally and externally photostandardizing chemical assays e.g. an ATP-bioluminescence assay. A pre-determined amount of a photosensitive derivative of the analyte of interest is used in the assay protocol which releases a known amount of the free analyte when it is exposed to a flash of visible light of pre-determined duration and intensity. By monitoring the response of a test property of the assay to the release of a known quantity of analyte, a standard value can be calculated which allows either direct determination of the amount of analyte originally present in the assayed sample or production of a photocalibration series against which results of an assayed sample can be compared.
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申请公布号 |
GB2301436(A) |
申请公布日期 |
1996.12.04 |
申请号 |
GB19960020993 |
申请日期 |
1995.04.06 |
申请人 |
* BRF INTERNATIONAL |
发明人 |
WILLIAM JOHN * SIMPSON;JULIAN M * PYE |
分类号 |
G01N33/50;C12Q1/66;G01N21/77;G01N21/78;G01N33/58;G01N33/96;(IPC1-7):C12Q1/66 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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