| 摘要 |
<p>PURPOSE:A human cultivated and established cell 'OC-1', obtained from primary culture of a human ovarian tissue and capable of producing a plasminogen activator in a culture medium with a high productivity and permanent cultivation. CONSTITUTION:A human cultivated and established cell obtained by subjecting a cultivated cell group derived from a human ovarian tissue to subculture isolating a single cell group having the high ability to produce a plasminogen activator (hereinafter abbreviated to PA). The cell is in the oval form with 20-80mum size, but sometimes assumes an amorphous form (the major axis/minor axis ratio is increased when the cell density is low, but close to spherical form in the case of single layer formation). The cell is capable of multiplying in Eagle MEM culture medium, and preservable at -196 deg.C for a long period. The ability to produce the PA is stable for a long period, and PA having about 50,000 daltons molecular weight and single enzyme activity ban d by enzymography can be produced in a culture medium with high productivity. The permanent cultivation can be carried out.</p> |
