| 摘要 |
Reagent for determining glucose, cholesterol, uric acid or haemoglobin comprises (1) 1-phenyl-2,3-dimethyl -4-aminopyrazol-5-one (I); (2) a phenol deriv.; (3) buffer soln.; (4) at least 1 stabiliser, and opt. (5) at least one detergent and/or (6) at least one enzyme catalyst. The new feature is that component (2) is thymol (II). Pref. (II) is present as a soln. in 1-4C alcohol, esp. EtOH. - (II) is 2.3-2.7, esp. 2.5 mM; (I) 0.6-1, esp. 0.8 mM and the buffer is aq. Na2HPO4-KH2PO4 at 0.06-0.1, esp. 0.08 M. The pref. stabiliser is NaN3 at 9.75-9.95, esp. 9.85 mM and the detergents are polyoxyethylene ether and/or polyalkanolalkyl ether at 0.2-1, esp. 0.4 wt.%. - The pref. enzymes are (A) glucose oxidase at 8-12, esp. 10, units/ml plus peroxidase at 13-17, esp. 15, units/ml; (B) cholesterinase at 8-12, esp. 10, units/ml; cholesterol oxidase at 4-6, esp. 5, units/ml and peroxidase as in A and (c) uricase at 4-6, esp. 5, units/ml and peroxidase as in A. The reagent pref. contains 310-350, esp. 330, mM alkanol.
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