发明名称 HETEROGENEOUS ASSAY METHOD FOR ANTIGENIC BIOLOGICAL SUBSTANCES USING ANTIBODY-HYBRID CONJUGATES, AND REAGENT THEREFOR
摘要 1. Claims (for the Contracting States : BE, CH, DE, FR, GB, IT, LI, LU, NL, SE) Heterogeneous phase assay method for antigenic substances, which consists in : a) reacting the sample containing the substance to be assayed with an antibody (or an antigen) immobilized on an insoluble matrix, b) bringing the matrix, which has reacted according to a), into contact with a coupling product composed of an antibody (or antigen) to the substance to be assayed and an anti-enzyme antibody bridged via symmetrical molecules chosen from benzoquinone and glutaraldehyde, which molecules are bound to them covalently, c) then reacting the enzyme corresponding to the antibody which composes the coupling product, and d) assaying the enzyme bound to the matrix modified during the stages a) to c). 1. Claims (for the Contracting State AT) Heterogeneous phase assay method for antigenic substances, which consists in : a) reacting the sample containing the substance to be assayed with an antibody (or an antigen) immobilized on an insoluble matrix, b) bringing the matrix, which has reacted according to a), into contact with a coupling product composed of an antibody (or antigen) to the substance to be assayed and an anti-enzyme antibody bridged via symmetrical molecules chosen from benzoquinone and glutaraldehyde, which molecules are bound to them covalently, c) then reacting the enzyme corresponding to the antibody which composes the coupling product, and d) assaying the enzyme bound to the matrix modified during the stages a) to c).
申请公布号 DE3461566(D1) 申请公布日期 1987.01.15
申请号 DE19843461566 申请日期 1984.03.14
申请人 INSTITUT PASTEUR 发明人 GUESDON, JEAN-LUC;AVRAMEAS, EUSTRATE
分类号 G01N33/531;G01N33/535;(IPC1-7):G01N33/543 主分类号 G01N33/531
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