PROCESS FOR THE QUANTITATIVE DETERMINATION OF THE MUTAGENICITY OF CHEMICAL AGENTS USING PHAGES IN AN IN VITRO TEST

摘要

1. A process for the quantitative determination of the mutagenic effect of chemical substances on phages by a test carried out in vitro, characterized in that for the statistic evaluation of the phages to a suspension of the phages having a concentration of 10**6 to 10**8 phages/ml prepared with a buffer solution having a pH of 6,0 to 8,0 a known amount of a chemical substance to be tested and being soluble in or mixable with water, utmost 20 % by volume, aqueous methanol or ethanol solution or utmost 38 %, by volume, aqueous dimethyl sulfoxid solution is added, as phages those are being used which comprise no lysogenic gene containing doublestranded DNS, then after the addition of the chemical substance immediately a sample is taken from the suspension and the starting phages concentration of the sample is determined by breeding and cultivation, respectively in a manner known per se on a corresponding host cell, and the phages suspension containing the chemical substance is maintained at a temperature of 20 to 37,5 degrees C, a sample is taken from the suspension form time to time and the concentration of the surviving phages is determined at the point of sampling by breeding and cultivation, respectively in a manner known per se on a corresponding host cell, and on the basis of the obtained data the numeric value being characteristic for the mutagenic effect is calculated.