Glycoconjugate vaccines

摘要

Glycoconjugate vaccines and methods of preparing and using the same are described.

主权项

1. A method of preparing a glycated peptide conjugate, the method comprising:
obtaining a population of polysaccharides; treating the population of polysaccharides to create a treated population of polysaccharides having an average molecular weight of 10-20 kDa; contacting the treated population of polysaccharides with polypeptides comprising a plurality of repeating peptide units linked together by cleavable moieties, wherein each peptide unit consists of: (i) an MHC-II binding sequence of 20 amino acids or fewer, wherein none of the amino acids are lysine; (ii) one lysine residue at the C terminal end; and (iii) one or more amino acids linking the MHC-II binding sequence to the lysine residue, wherein none of the amino acids are lysine; under conditions sufficient to link the treated polysaccharides directly to the lysine residues, thereby preparing the glycated peptide conjugate.