| 摘要 |
PURPOSE:To obtain the titled nucleic acid probe to form stable complex with RNA or DNA, by bonding successively 5'-OH and 3'-OH of riboside such as 2'-O-methyluridine, etc., through phosphoric acid and removing the protecting group. CONSTITUTION:5'-OH and 3'-OH of a riboside selected from 2'-O-methyluridine, 2'-O-methyl-N<4>-protected cytidine, 2'-O-methyl-N<6>-protected-adenosine and 2'-O- methyl-N<2>-protected guanosine are successively bonded (condensed) through phosphoric acid by phosphoric acid triester method or phosphorus acid triester method in such a way that preset sequence (e.g., sequence of partial structure of messenger RNA, etc.) order is obtained and the protecting group is removed to give the aimed compound. The condensation reaction is carried out on a solid-phase carrier such as silica gel, polystyrene, porous glass, etc., and the reaction product is separated from the carrier after the condensation reaction is over, isolated and purified by column chromatography, etc. |
