发明名称 |
Method for isolating a chemotherapeutic agent resistant cancer cell with stem cell properties |
摘要 |
The invention relates to the use of encapsulates of cancer cells, in agarose coated, agarose containing beads, for isolating chemotherapeutic resistant cells which have at least one stem cell property, such as expression of OCT4. The cells thus isolated are also a feature of the invention, as is a method for screening for potential therapeutic agents. |
申请公布号 |
US9375448(B2) |
申请公布日期 |
2016.06.28 |
申请号 |
US201414262232 |
申请日期 |
2014.04.25 |
申请人 |
THE ROGOSIN INSTITUTE, INC. |
发明人 |
Gazda Lawrence;Smith Barry |
分类号 |
C12N5/09;A61K33/24;C12N5/095;G01N33/543;A61K31/282;A61K31/337;A61K31/437;A61K31/519;A61K31/704 |
主分类号 |
C12N5/09 |
代理机构 |
Abelman, Frayne & Schwab |
代理人 |
Abelman, Frayne & Schwab |
主权项 |
1. A method for determining prognosis of a subject suffering from cancer, comprising:
(i) encapsulating a sample of cancer cells taken from said subject in an agarose containing, agarose coated bead; (ii) contacting said agarose containing, agarose coated bead with an anti-cancer agent known to be effective against said cancer cells, said anti-cancer agent being contacted to said bead at a concentration known to be effective, for a period of time known to be effective; (iii) determining number of cancer cells remaining in said bead after the period of time of (ii); and (iv) wherein a high number of said cancer cells as compared to a patient with a good prognosis is indicative of a poor prognosis for said subject. |
地址 |
New York NY US |