| 摘要 |
<p>Coniferous trees, such as European larch and jack pine, are clonally micropropagated by germinating surface-sterilized seeds on translucent, gel-solidified water, removing the seed coat from the germinated seeds, separating cotyledons and hypocotyls from the embryonic tissue and incubating same on a gel-solidified bud initiation medium containing about 5 to about 15 mg/l of a cytokinin, such as benzylaminopurine, and 0 to about 0.01 mg/l of an auxin, such as naphthalene acetic acid, to promote the formation of adventitious buds, transferring the budded tissue to a bud development medium free of plant growth regulators and containing activated carbon to promote the growth of the adventitious buds, transferring the budded tissue to a shoot elongation medium free of plant growth regulators and activated carbon to promote growth of shoots to a length suitable for initiation of root growth, and separating shoots and introducing individual shoots into a nutrient medium for initiating root growth. In one embodiment, needles and stems from plantlets grown from cotyledons and hypocotyls separated from embryonic tissue are recycled for use as source tissue which is incubated in the bud initiation medium to produce subsequent and successive generations of plantlets.</p> |
