发明名称 Preparation of protein G and/or fragments thereof
摘要 <p>@ Hybrid-DNA-technique for the manufacture of protein G and/or fragments of protein G with substantially the same properties as protein G insofar as the capability of binding IgG is concerned.</p><p>In accordance with the invention a hybrid-DNA-molecule is produced comprising a vector into which has been introduced a DNA-sequence deriving from streptococci which codes for protein G and/or the said fragments thereof.</p><p>In accordance with the invention, moreover, a micro-organism is produced belonging, for example, to a strain of E.coli which has been transformed by a hybrid-DNA-molecule in accordance with the above, and a method for preparing the transformed micro-organism.</p><p>Finally, a method is provided for the preparation of protein G and/or the said fragments thereof, according to which a micro-organism which has been transformed by the said hybrid-DNA-molecules cultivated in a suitable culture medium and the protein G product is isolated.</p>
申请公布号 EP0200909(A2) 申请公布日期 1986.12.17
申请号 EP86104244.8 申请日期 1986.03.27
申请人 PHARMACIA BIOSYSTEMS AB 发明人 BJOERCK, LARS;KRONVALL, GOERAN;LINDAHL, GUNNAR;KASTERN, WILLIAM
分类号 (IPC1-7):C12N15/00;C12P21/00;C12N1/20;C07H21/04 主分类号 (IPC1-7):C12N15/00
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