Method of preparing a reaction mixture and related products

摘要

The invention relates to a method of preparing a reaction mixture for Polymerase Chain Reaction (PCR) assay and a solution set for PCR. The method comprises providing a sample solution comprising a biological sample to be amplified in said PCR assay and first colorant providing the solution a first color, providing a reagent solution comprising at least one other substance required for performing said assay and second colorant providing the solution a second color different from the first color, and mixing the sample solution and the first reagent solution for providing a mixed solution to be subjected to the PCR process, the mixed solution having, due to said first and second colorants, a third color different from the first and second colors. The invention significantly aids in pipetting PCR assays.

主权项

1. A solution set for a polymerase chain reaction (PCR) assay, the solution set comprising
a first reagent solution comprising
a first colorant having a first color distinguishable in the visible spectrum, anda sample solution containing a template to be amplified, and a second reagent solution comprising
a second colorant having a second color distinguishable in the visible spectrum, the second color different from the first color, andat least one substance selected from the group consisting of a polymerase, a primer, a plurality of dNTPs, a fluorescent qPCR dye, and a probe,at least the first and second reagent solutions on mixing forming a mixed solution, the mixed solution having, due to the first and second colorants, a third color different from the first and second colors and distinguishable in the visible spectrum.