TaBV transcriptional control element, chimeric constructs and uses therefor

摘要

This invention discloses a constitutive promoter for expression of foreign or endogenous coding sequences in plants, including dicotyledonous and monocotyledonous plants. The invention also discloses a chimeric nucleic acid construct comprising the promoter of the invention operably linked to a foreign or endogenous polynucleotide that codes for a protein of interest or a transcript capable of modulating expression of a target gene. The invention further discloses transformed plant cells, as well as differentiated plants and plant parts, containing the construct. Methods for diagnosis and treatment of viral infections, especially badnaviral infections, are also disclosed.

主权项

1. A chimeric DNA construct comprising a nucleotide sequence having promoter activity and operably linked to a foreign or endogenous DNA sequence to be transcribed, wherein the nucleotide sequence hybridizes under high stringency conditions to the sequence set forth in any of one of SEQ ID NO:6, 7 or 8, and wherein the high stringency condition is 1% BSA, 1 mM EDTA, 0.5 M NaHPO4 (pH 7.2), and 7% SDS for hybridization at 65° C. and washing at 68° C. in 0.2×SSC, 0.1% SDS.