Method and device for detection and quantification of analytes

摘要

The invention describes a device for detecting and quantifying an analyte in a sample and a method of using said device, the device comprising a disposable cartridge (8) comprising a microfluidic inlet (1) for loading the sample comprising reporter probe - analyte - capture probe - magnetic bead complexes; a purifying chamber (4) for purifying the magnetic bead complexes; a measure chamber (5) comprising a chip (6) comprising electrodes; external electromagnets (13, 13') situated each on opposite sides of the purifying chamber (4) for retaining, or releasing or shaking magnetic bead complexes present in the purifying chamber (4); a permanent magnet (14) located under the working electrode of the measurement chamber (5), to attract and retain the magnetic bead complexes on the surface of the working electrode of the chip (6); and a potentiostat (15) for carrying out the electrochemical detection and quantification of the analyte.