| 摘要 |
<p>PURPOSE:To produce hepatitis B virus (HBV) protein, by integrating two fragments of hepatitis B virus gene, cleaved by BamHI and connected in series at the cleavage sites thereof between the cleavage sites of a specific shuttle vector cleaved by the BamHI. CONSTITUTION:A recombinant DNA integrating two fragments of hepatitis B virus gene, cleaved by BamHI and connected in series at the cleavage sites thereof between the cleavage sites of a shuttle vector capable of multiplying in both Escherichia coli and animal cells is prepared. The resultant recombinant DNA is then introduced into Vero cells to prepare transformant Vero cells, which are cultivated in a culture medium for animal cells to produce hepatitis B virus surface antigen, surface antigen with pre S and HBe antigen proteins in the resultant culture. The resultant proteins are then recovered to afford the aimed hepatitis B virus protein.</p> |
