| 摘要 |
NEW MATERIAL:An antichondroitin 6 sulfate monoclonal antibody produced from a hybridoma formed by cell fusion of a preimmunized mouse lymphpocyte using proteochondroitin sulfate derived from a tissue containing chondroitin 6 sulfate as an antigen and mouse myeloma. USE:For diagnosing a malignant tumor by detecting chondroitin 6 sulfate. PREPARATION:For example, proteochondroitin derived from a tissue containing chondroitin 6 sulfate is used as an antigen, administered into a mouse, the mouse is immunized against it, a lymph node, etc., is extracted, to collect lymphocyte. Then, the lymphocyte and myeloma cell of mouse are subjected to cell fusion, formed hybridoma is cloned by limiting dilution method and it is cultivated to give a monoclonal antibody.
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