| 摘要 |
PURPOSE:To easily and accurately measure concn. in a specimen, by forming calibration curves over all of regions containing an antigen excessive region and an antibody excessive region with respect to agglutination reaction times T1, T2 to calculate the agglutination ratios of a body fluid specimen at reaction times T1, T2 and setting the concn. value common to both reaction times to the concn. of an antigen (antibody) from both calibration curves. CONSTITUTION:A definite amount of latex particles used in the sensitization of an antibody is added to a body fluid specimen and antigen-antibody agglutination ratios to the concn. of an antigen are calculated at agglutination reaction times T1 (20sec), T2 (300sec) over all of regions containing an antibody excessive region A and an antigen excessive region B according to formula [wherein Pi is the number (i) of agglutinated particles, Wi is the weighing coefficient added to the number (i) of agglutinated particles, Pj is the number (j) of agglutinated particles and Wj is the weighing coefficient thereof] to form calibration curves. The agglutination ratio of the body fluid specimen and a definite amount of an antibody comes to a3 at the reaction time T2 and, when two concn. values C1, C2 are determined from the calibration curves, an agglutination ratio is calculated at the reaction time T1 and the concn. value C2 wherein the value a2 thereof is the same concn. as a3 is set to the concn. of the antigen in the specimen. By this method, the necessity of the re-dilution and re-measurement of the specimen is eliminated to attain accurate measurement. In the same way, the concn. of the antibody can be measured.
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