摘要 |
Diazonium salts which are useful in an assay for bilirubin are disclosed. The novel salts have the structural formula: <IMAGE> wherein X- is a stabilizing anion, Y is CO- or SO2-; and R1 and R2 are independently selected from hydrogen, alkyl, aryl, carboxyalkyl and hydroxyalkyl providing R1 and R2 are not both hydrogen; R3 and R4 are independently selected from groups such that the sum of the Hammett sigma values for R3 and R4 does not exceed +0.4 or R3 and R4 represent the carbon atoms necessary to complete a fused carbocyclic ring structure. Reagent compositions, analytical elements and methods which use the described diazonium salt are also disclosed. |