| 摘要 |
<p>PURPOSE:To enable the efficient proliferation of cells and to maintain and culture the cell in high density, by using a membrane permeable to the nutrient necessary for the proliferation of the cell and impermeable to the cell and culturing the cell while circulating the suspension of the cell or the culture liquid. CONSTITUTION:The apparatus is provided with hollow fibers having wall membrane permeable to the nutrient necessary for the proliferation of the cell and impermeable to the cell. The culture liquid is sent from the culture liquid tank 8 with the pump 9 to the cell culture apparatus 10 in which the cells are cultured in the space 6 between the above hollow fibers and/or outside of the hollow fibers. The cell suspension or culture liquid between the fibers is circulated with the pumps 11, 12 through the circuits 13, 14 and the space 6.</p> |
