<p>The 5-substituted hydantoins represented below can be transformed into D-α-amino acids by the use of the cultured broth, cells or treated cells of the genus Hansenula.</p><p>5-substituted hydantoins:<!-- EPO <DP n="2"> --><Chemistry id="chema01" num="0001"><Image id="ia01" he="47" wi="66" file="IMGA0001.TIF" imgContent="chem" imgFormat="TIFF" inline="no" /></Chemistry>wherein R represents an alkyl, substituted alkyl, phenyl or substituted phenyl group. D-a-amino acids: R-CH-COOH NH<Sub>2</Sub> wherein R represents the same meanings as above.</p>