| 摘要 |
PURPOSE:To obtain a sharp peak having no tailing, by using a buffer solution with pH 4 or more, which arbitrarily contains an org. solvent containing an alkaline earth metal ion and capable of being mixed with water, as the mobile phase in chromatography. CONSTITUTION:The titled analytical method is constituted by subjecting a specimen capable of containing tetracycline to high performance liquid chromatography and, as the mobile phase in the chromatography, a buffer solution with pH 4 or more, which arbitrarily contains an org. solvent containing an alkaline earth metal ion and capable of being mixed with water, is used. By adding the alkaline earth metal ion, a chelate is positively formed between said ion and tetracyclines and tetracyclines are separated and analyzed in a chelate form by chelate forming reaction. That is, because the component to be analyzed is already formed into a chelate, said chelate is eluted without generating trouble such that said component forms a chelate along with the metal contained in an outflow wall or the packing agent of the column to be adsorbed and a sharp peak having no sharp tailing is imparted.
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