摘要 |
PURPOSE:To produce an immobilized alcohol oxidase, useful mainly for electrodes, easily operable, and having good stability, by incorporating polyethylenimine and glutaraldehyde in specific concentrations in an enzymic solution. CONSTITUTION:1-7vol%, preferably 4-6vol% polyethylenimine and 1-3vol% glutaraldehyde are incorporated in an enzymic solution consisting of a buffer solution of 6-8pH, preferably 7.0-7.5pH used for immobilizing an alcohol oxidase to crosslink and immobilize mutually the alcohol oxidase and glutaraldehyde. |