| 摘要 |
PURPOSE:To determine both a diamine and a polyamine by a simple device by a simple operation in a short time, by treating a test specimen with a di- and/or polyamine oxidase, treating formed H2O2 with catalase in the presence of an alcohol and measuring the formed aldehyde. CONSTITUTION:A test specimen is treated with a diamine oxidase (e.g., produced by germinated soybean or by organisms) and/or a polyamine oxidase (e.g., produced by animal source such as bovine plasma, etc.). Then formed H2O2 is treated with a catalase (e.g., preferably produced by animal source) in the presence of an alcohol such as methanol, ethanol, etc., the formed aldehyde is measured to determine a diamine and a polyamine. The measurement of the aldehyde is carried out by chemical method wherein direct colorimetry is used. The determination of this invention is applicable to an automatic analyzer, so it an be adopted widely to day-to-day, clinical diagnosis method and utilized for detecting, diagnosing and remedying cancers.
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