| 摘要 |
PURPOSE:To produce, a large amount of L-histidine inexpensively, by cultivating cultivating a specific Coryneform mold capable of producing histidine in a mold. CONSTITUTION:A bacterium (e.g., Corynebacterium glutamicum GAUPTr-113) capable of producing L-histidine is varied with nitrous acid, to give a variant (e.g., Corynebacterium glutamicum Re-13) which has no ability or extremely reduced ability to grow using D-ribose as the only carbon source. Then, it is aerobically cultivated in a medium consisting of a carbon source (e.g., molasses), a nitrogen source (e.g., urea), an inorganic salt (e.g., NaCl), etc., at 20-40 deg.C at 5-9pH for 2-7 days, and L-histidine is accumulated in the culture mixture. Then, it is isolated by ion exchange resin method, etc.
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