| 摘要 |
PURPOSE:To detect a cancer-associated antigen with good sensitivity by crushing cells to liberate the cancer-associated antigen or the antigen in the cells and detecting the liberated antigen. CONSTITUTION:A pancrea structure lump 1 is cleaned with a Hanks soln. and is transferred into a petri disch 2. The lump is then cut to fragments by a surgeon's knife or scissors. The tissue fragments 3 thereof are transferred into a flask 4 contg. a disperse or trypsine liquid 5 and the liquid is shaken. The enzyme liquid 5 is removed by centrifugal sepn. or filtration, by which the suspension 6 of the cells is obtd. Antigen CEA8a exists on the film surface of the cancer cells 7 and the antigen is produced in the cells as well if the cells are cancerized. An antigen CEA antibody 9 is added to the liquid 6 to induce the antigen antibody reaction with all of the CEA on the film. The antibody 9 is preliminarily labeled by FITC10. The unreacted FITC-anti-CEA antibody is then removed. The cells 7 are crushed by a blender and the antigen is dissolved in a nonionic surface active agent soln. The soln. is passed through a solid phase 12 bound with an anti-rabbit IgG antibody 11 and the fluorescence of the solid phase 12 is measured.
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