| 摘要 |
PURPOSE:The titled substance obtained by treating microbial cells of Streptococcus faecium with hot water, and salting out the resultant extract. CONSTITUTION:An antitumor active substance obtained by cultivating a strain, e.g. Streptococcus faecium R 672 strain; FERM-P No.8167) belonging to Streptococcus faecium, and collecting the microbial cells from the culture by the centifugation, washing the micobial cells with physiological saline solution, etc., adding an adequate amount of purified water thereto, heating the resultant mixture, and extracting the substance with hot water. The amount of the purified water to be used for extraction with the hot water is 5-50 times, preferably 8-15 times based on the wet weight of the microbial cells. The extraction temperature is 4-100 deg.C. A salt, e.g. ammonium sulfate, is used to salt out the extract, and deposited precipitate is separated by filtration or centrifugation, etc., and the resultant residual salt is removed by dialysis, gel filtration, etc. The resultant precipitate is then freeze-dried to give the aimed antitumor active substance. The preferred dosage form is injection and transfusion solution, and the dose thereof is 1-2,000mg/day. |
