| 摘要 |
PURPOSE:To enable analysis of a specified object to be inspected with a simple and small apparatus without any B.F separation (separation of a labeled antigen or antibody involved in antigen-antibody reaction from those not involved therein), by sucking a free labeled substance after an emulating reaction into a flowcell to make an enzyme reaction. CONSTITUTION:A specific amount of an antibody 2 which is subjected to an antigen-antibody reaction specifically with alpha-fetoprotein (AFP) is arranged on the inner wall of a reactor 1 as converted to a solid phase, a serum sample is injected into the container 1 and the AFP3 done into a specified amount of a labeled reagent with luciferine 4 and the AFP5 in the sample and the AFP3 labeled with luciferine 4 in the labeled reagent are subjected to a emulating reaction with the antibody 2. The, the reaction liquid in the container 1 is sucked into a flowcell 7 having luciferase 6 converted to a solid phase. Thus, the AFP5 and the AFP3 not involved in the antigen-antibody reaction are sucked into the flowcell 7, where the luciferine 4 bonded to the AFP3 is oxidized with the luciferase 6 as catalyst to emit light. The amount of the emission is received with a photodetector 8 to identify and assay the AFP5.
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