摘要 |
PURPOSE:To easily reproduce an eluate and monitor composition by reproducing the eluate by film separation and monitoring the composition of the eluate by detecting and controlling it with the eluation pattern of a target component. CONSTITUTION:Mixed liquid which contains a material to be refined is added at the upper part of a column 5 and the eluate is sent from an eluate storage tank 1 to a line mixer 3 through a valve 2 by a high pressure pump 4, mixed, and fed to the column 5. A difference in moving speed is generated in the column 5 owing to the material and materials which are eluated are detected by a detector 6 in order of eluation; and a valve 7 is opened on the basis of previously inputted information to gather fractions containing only the target material to be refined by a collector 8. Compatible materials are removed by a film module 9 from an eluate which contains materials other than the target material and the eluate is adjusted to initial eluate composition and sent to the column 5 again by the pump 4 to circulate repeatedly. The eluation time of the target material from the column is inputted to a computer previously and if a time difference is generated, a valve 2 is opened to control the composition of the eluate automatically. |