| 摘要 |
PURPOSE:To dissociate an antibody with a high dissociation ability, by mixing a sample blood with a specific dissociating solution in blood transfusion, subjecting the resultant mixture solution to centrifugal separating operation to separate layers, and taking out only the antibody layer, floating on the topmost layer, and dissolved therein. CONSTITUTION:A given amount of blood is taken from a collected blood, and an antibody is dissociated from erythrocytes therein. In the process, the sample blood is mixed with physiological saline solution and a dissociating solution consisting of trichlorotrifluoroethane and methylene chloride (a mixture of the former with the latter at 95-20:5-80, preferably 30:70 ratio) are in equivalent volumes, and the resultant mixture solution is then subjected to centrifugal separating operation (under conditions of 30-40 deg.C temperature, 2,500-3,500r.p.m. for 3-7min) to separate layers. Only the antibody layer, floating on the topmost layer, and dissolved therein is taken out to separate the antibody from erythrocytes. According to this method, a very high dilution ratio can be obtained in separating the antibody, and the dissociating operation can be rapidly carried out while removing chronic poisoning of operators by organic solvents. |
