| 摘要 |
PURPOSE:To produce a sugar containing a remarkable amount of fructooligosaccharide, by treating a particular sugar substrate with extracellular enzyme of a specific Bacillus strain. CONSTITUTION:An alkalophilic microbial strain belonging to Bacillus genus and having optimum pH for growth at neutral and alkaline regions is selected, and cultured aerobically in a liquid medium composed of sucrose, polypeptone, etc., at 35-55 deg.C for 20-50hr to effect the production and accumulation of crude fructosyl transferase in the supernatant liquid of the culture liquid (extracellular). The crude enzyme is separated and purified to obtain the enzyme (A) having the physico-chemical and enzymo-chemical properties of the Table. The component A is added to a liquid containing 50-70w/v% sugar substrate (B) (e.g. sucrose) having beta-D-fructofuranoside bond, and the sugar-transfer reaction is carried out at 50 deg.C to produce a fructo-oligosaccharide. The produced oligosaccharide is separated by column chromatography using a gel-filtration resin, etc. |
